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   식충식물 중에서 대표적인 식충식물인 끈끈이주걱과 토종 끈끈이귀게 그리고 파리지옥의 조직배양 과정을 아래에 설명하였으며 자세한 내용은

  본 연구소 소장의 논문

 

  (1) 연구소에서 육종한 파리지옥 신품종(Korea melody shark)을 미국 ICPS에 국내 최초로 품종등록
               
       Volume 39, No.2  2010

  (2) Micropropagation of Drosera peltata, a tuberous sundew, by shoot tip culture (토종 끈끈이귀개의 줄기배양에 의한 급속 대량 증식 )

       Plant Cell, Tissue and Organ Culture 77 : 211-214, 2004을 참고하길 바란다.

  (3) Micropropagation of Venus fly trap by shoot culture (파리지옥의 줄기배양에 의한 급속 대량 증식)

       Plant Cell, Tissue and Organ Culture 72 : 95-98, 2003을 참고하길 바란다.

  (4) Mass   Propagation of Sundew, Drosera rotundifolia L. through Shoot Culture (끈끈이주걱의 줄기배양에 의한 급속 대량 증식)

      J. Plant Biotechnology 1 : 97-100, 1999

 

                                 - (1) Dionaea 'Korean melody shark'  -

 

Dionaea ‘Korean Melody Shark’

Submitted: 26 March 2010

This cultivar was produced by crossbreeding different clones of plants known informally in the UK

as Dionaea “Shark Tooth”. The crossbreeding was done in the Korean Carnivorous Plant Institute by

the head of the institute Dr. Jang Gi-Won and his intern Max Yoon.

The crossbreeding was not done with the intention of producing a new cultivar. Normally work at

our Institute focuses on conservation. We are currently involved in a project to prevent certain Korean

carnivorous plants from extinction. For instance, Drosera rotundifolia L. once lived in many regions

of Korea. However, due to mass construction and exploitation without awareness of the importance

of conserving carnivorous plants, many natural habitats have been destroyed. Therefore, in order to

conserve Korean carnivorous plants, we have practiced mass propagation techniques of carnivorous

plants. This new Dionaea cultivar was one unexpected result of our work.

By germinating 100 seeds after asepsis, one plant appeared to be different from the other ninety

nine. We separated this unique plant and mass propagated it. It seems like this cultivar, which we named

Dionaea ‘Korean Melody Shark,’ is a sterile mutant—after growing the cultivar and finally seeing its

flower, we discovered that the pistil and stamen do not reach maturity. Therefore, we could not collect

any seeds. It is thus only possible to multiply the cultivar by dividing the rhizomes.

 


Figure 14: Dionaea ‘Korean Melody Shark’.


Dionaea ‘Korean Melody Shark’ and its parent plants are similar, but there are some important

differences. First, the petiole of Dionaea ‘Korean Melody Shark’ remains very thin and narrow

throughout (see Figure 14). Traps that are not yet fully grown look like bean sprouts. When the traps

are fully grown, they become broader in shape. The sawteeth along the trap margin of Dionaea ‘Korean

Melody Shark’ are broader, shorter, and are arranged in a irregular pattern compared to those of its

parents (see Figures 15 and 16). Finally, while the parent plants remain green throughout, the inside of

the traps of Dionaea ‘Korean Melody Shark’ are red.

 



Figure 15: Dionaea ‘Korean Melody Shark’ trap.

 

Figure 16: Parent plants of Dionaea ‘Korean Melody Shark’ from the UK informally named Dionaea “Shark Tooth”.

 

We named this cultivar “Korean” because it came from Korea, where this new carnivorous plant

cultivar has been named, “Melody” because the traps look like musical notes, and “Shark” as an allusion

to the informal name of the parent plants.

— Dr. Jang Gi-Won • Korean Carnivorous Plant Institute • 107-6, SoungNae-Dong, GangDong-Gu

• Seoul, 134-030 • Republic of Korea • jgw77@yahoo.co.kr

— Wook Hyon (Max) Yoon • Jinhung Apartment 7 Dong 104 Ho Gangnam-gu • Chungdam-dong

• Seoul, 135-100 • Republic of Korea • mylifestyle_3@hotmail.com

 


 

                                 - (2) 토종 끈끈이귀개의 조직배양 과정 -

 

    Micropropagation of Drosera peltata, a tuberous sundew, by shoot tip culture

    (토종 끈끈이귀개의 줄기배양에 의한 급속 대량 증식 )

     출처 : Plant Cell, Tissue and Organ Culture 77 : 211-214, 2004. Gi-Won Jang

     Abstract

    In order to establish and optimize an in vitro micropropagation method of Drosera peltata (a tuberous sundew), a carnivorous

    plant, the effects of medium type, MS medium concentration, pH, and cytokinin type on shoot proliferation and tuber formation

    were investigated, using one-month-old shoot tips. The shoot proliferation and tuber formation were most effective on 1/2MS medium

    without cytokinins. The optimum pH of the media was pH 5.7. Tubers were planted in plastic pots filled with 1:1 peat moss and sand.

    The survival rate of the plantlets was almost 100%, and they exhibited normal development. With subculture every 12 weeks,

    hundreds of the plants were propagated from a single plant within a year.


 

                                   - (3) 파리지옥의 조직배양 과정 -

 

    Micropropagation of Venus fly trap by shoot culture (파리지옥의 줄기배양에 의한 급속 대량 증식)

     출처 : Plant Cell, Tissue and Organ Culture 72 : 95-98, 2003. Gi-Won Jang

     Abstract

    In order to establish and optimize an in vitro micropropagation protocol of Venus fly trap (Dionaea muscipula Ellis), a carnivorous

    plant, the effects of medium type, MS medium concentration, pH, and cytokinin and auxin types on shoot proliferation and root

    formation were investigated using 3-month-old shoots. The shoot proliferation was most effective in 2.3 μM kinetin-supplemented

    1/3MS medium at pH 5.5. The best conditions for rooting were 1/3MS medium supplemented with 0.5 μM IBA. All subcultured shoots

    produced extensive root systems after 5~6 weeks culture. When plantlets after rooting were planted in plastic pots filled with

    1:1 peat moss and sand, the survival rate of plantlets was almost 100%, exhibiting normal development. With subculture every 8 weeks,

    hundreds of the plants were propagated from a single plant within a year.


 

                                   - (4) 끈끈이주걱의 조직배양 과정 -

     출처 : J. Plant Biotechnology 1 : 97-100, 1999. Gi-Won Jang

 

0주

3주

15주

23주

24주

종자발아

육묘

대량증식

순화

육묘


종자 또는 엽

무균 치상-MS배지,

1/2MS배지
 


MS배지 또는

1/2MS배지,

3% sucrose,

0.2% Gelrite,

pH5.5
 


1/2MS배지,

3% sucrose,

0.2% Gelrite,

pH5.5
 


외부의 밝은 장소
 


수태 또는 마사와

피트모스

1:1 혼합토양
 


1.05%NaOCl과

0.01% Tween-20의

혼합용액 : 5- 20분

소독
 


* 발아한 묘를

약 10- 12주간

배양한다.

* 엽에서 줄기와

뿌리 발생
 


묘의 잎 또는 줄기를

치상한다. 잎을 치상

할 때는 잎 뒷면이

배지에 붙게 해야한다.
 


대량증식된 묘가 든

프라스크를

외부환경에

적응시킨다.
 


잘 순화된 묘를

프라스크에서 꺼내

심은 후 습도가

약 70% 이상 유지되는

장소에 약 1-2주

순화시킨 다음

외부환경에서

재배한다.
 

 

 

                        

                                            < 토종 끈끈이주걱의 엽 배양에 의한 유묘 발생 >

    E-mail  :  jgw778@naver.com

 

Copyright ⓒ 2002 Jang Gi-Won. All rights reserved